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Mkl1 Antibody (CL4281) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44096

Novus Biologicals, part of Bio-Techne
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NBP3-44096

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4281

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q969V6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Mkl1 Antibody (CL4281) - Azide and BSA Free (NBP2-59047) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Mkl1 Antibody (CL4281) - Azide and BSA Free

Mkl1 Antibody (CL4281) - Azide and BSA Free Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP3-44096]

Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP3-44096]

Staining of human breast shows moderate nuclear positivity in a subset of glandular cells.
Mkl1 Antibody (CL4281) - Azide and BSA Free Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP3-44096]

Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP3-44096]

Staining of human liver shows no positivity in hepatocytes as expected.
Mkl1 Antibody (CL4281) - Azide and BSA Free Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP3-44096]

Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP3-44096]

Staining of human lymph node shows moderate cytoplasmic and nuclear positivity in germinal center cells.

Applications for Mkl1 Antibody (CL4281) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mkl1

MKL1 (megakaryoblastic leukemia 1 protein) was identified as part of a t(1;22) translocation with RBM15 associated with acute megakaryoblastic leukemia. It is a transcriptional coactivator of serum response factor (SRF) that promotes muscle differentiation and also plays a role in promoting maturation of megakaryocytes.

Long Name

Myocardin-related transcription factor A

Alternate Names

KIAA1438, MAL, MRTF-A, MRTFA

Gene Symbol

MRTFA

Additional Mkl1 Products

Product Documents for Mkl1 Antibody (CL4281) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mkl1 Antibody (CL4281) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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