MISP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14390
![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0002.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: SQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MISP1 Antibody - BSA Free (NBP2-14390) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MISP1 Antibody - BSA Free
![Western Blot: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Western-Blot-NBP2-14390-img0009.jpg "Western Blot: MISP1 Antibody [NBP2-14390]")
Western Blot: MISP1 Antibody [NBP2-14390]
Western Blot: MISP1 Antibody [NBP2-14390] - Analysis in control (vector only transfected HEK293T lysate) and MISP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14390-img0008.jpg "Immunocytochemistry/ Immunofluorescence: MISP1 Antibody [NBP2-14390]")
Immunocytochemistry/ Immunofluorescence: MISP1 Antibody [NBP2-14390]
Immunocytochemistry/Immunofluorescence: MISP1 Antibody [NBP2-14390] - Staining of human cell line U-2 OS shows localization to plasma membrane and focal adhesion sites.![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0015.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]
Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human liver shows no positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0005.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0006.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0007.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0011.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0012.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0013.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
![Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]](https://resources.bio-techne.com/images/products/MISP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14390-img0014.jpg "Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]")
Applications for MISP1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:200-1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MISP1
Alternate Names
chromosome 19 open reading frame 21, DKFZp686H18209, hypothetical protein LOC126353
Gene Symbol
MISP
Additional MISP1 Products
Product Documents for MISP1 Antibody - BSA Free
Product Specific Notices for MISP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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