Mesothelioma Antibody (ME1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22442

Novus Biologicals, part of Bio-Techne
Discontinued Product
NBP2-22442 has been discontinued. View all Mesothelioma products.

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Radioimmunoassay, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ME1

Format

BSA Free

Concentration

LYOPH mg/ml

View all Mesothelioma Antibodies »

Product Specifications

Immunogen

Mesothelioma cell line SPC111.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Mesothelioma Antibody (ME1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Mesothelioma Antibody (ME1) - BSA Free [NBP2-22442]

Immunocytochemistry/ Immunofluorescence: Mesothelioma Antibody (ME1) - BSA Free [NBP2-22442]

Immunocytochemistry/Immunofluorescence: Mesothelioma Antibody (ME1) [NBP2-22442] - Analysis of Mesothelioma in MCF-7 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Mesothelioma monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Mesothelioma staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Applications for Mesothelioma Antibody (ME1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

2 - 5 ug/ml

Immunohistochemistry-Paraffin

2 - 5 ug/ml

Radioimmunoassay

1:100 - 1:2000
Application Notes
Detects a surface membrane glycoprotein preferentially expressed on normal and malignant mesothelial cells from human tissues. Does not detect most lung adenocarcinoma cell lines, or well and moderately differentiated pulmonary adenocarcinomas. Does not work in WB. IHC: Staining of sarcomatoid mesotheliomas with this antibody results in intense staining of mesothelial cells; it will not react with formalin-fixed, or alcohol-fixed, paraffin embedded tissues. Use of frozen, acetone fixed sections or cell suspensions is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with PBS.

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mesothelioma

Mesothelioma is a rare form of carcinoma of the mesothelial cells which that normally line the body cavities, including the pleura, peritoneum, pericardium, and testis. It may be localized or diffuse. Most, but not all, cases of pleural malignant mesothelioma are associated with asbestos exposure.

Alternate Names

Malignant mesothelioma, susceptibility to

Gene Symbol

MMS

Additional Mesothelioma Products

Product Documents for Mesothelioma Antibody (ME1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mesothelioma Antibody (ME1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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