MEK5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89655
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MEK5 Antibody - BSA Free (NBP1-89655) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MEK5 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: MEK5 Antibody [NBP1-89655]](https://resources.bio-techne.com/images/products/MEK5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89655-img0006.jpg "Immunocytochemistry/ Immunofluorescence: MEK5 Antibody [NBP1-89655]")
Immunocytochemistry/ Immunofluorescence: MEK5 Antibody [NBP1-89655]
Immunocytochemistry/Immunofluorescence: MEK5 Antibody [NBP1-89655] - Staining of human cell line A-431 shows localization to nucleoli & microtubules. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: MEK5 Antibody [NBP1-89655]](https://resources.bio-techne.com/images/products/MEK5-Antibody-Immunohistochemistry-Paraffin-NBP1-89655-img0002.jpg "Immunohistochemistry-Paraffin: MEK5 Antibody [NBP1-89655]")
Immunohistochemistry-Paraffin: MEK5 Antibody [NBP1-89655]
Immunohistochemistry-Paraffin: MEK5 Antibody [NBP1-89655] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.![MEK5 Antibody - BSA Free Western Blot: MEK5 Antibody - BSA Free [NBP1-89655]](https://resources.bio-techne.com/images/products/nbp1-89655_rabbit-polyclonal-mek5-antibody-15520258221019.jpg "Western Blot: MEK5 Antibody - BSA Free [NBP1-89655]")
Western Blot: MEK5 Antibody - BSA Free [NBP1-89655]
Analysis in control (vector only transfected HEK293T lysate) and MAP2K5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Applications for MEK5 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MEK5
Long Name
Mitogen-activated Protein Kinase Kinase 5
Alternate Names
MAP2K5, MAPKK5, MKK5, PRKMK5
Gene Symbol
MAP2K5
Additional MEK5 Products
Product Documents for MEK5 Antibody - BSA Free
Product Specific Notices for MEK5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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