MEK1/2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05651
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAP2K1 (NP_002746.1). SRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSY
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MEK1/2 Antibody - BSA Free (NBP3-05651) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MEK1/2 Antibody - BSA Free
![Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]](https://resources.bio-techne.com/images/products/MEK1-2-Antibody-Western-Blot-NBP3-05651-img0002.jpg "Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]")
Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]
Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.![Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]](https://resources.bio-techne.com/images/products/MEK1-2-Antibody-Western-Blot-NBP3-05651-img0001.jpg "Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]")
Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]
Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of PC-12 cells, using MEK1/2 antibody (NBP3-05651) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Applications for MEK1/2 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:100 - 1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MEK1/MEK2
MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Symbol
MAP2K1
Additional MEK1/MEK2 Products
Product Documents for MEK1/2 Antibody - BSA Free
Product Specific Notices for MEK1/2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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