MEK1/2 Antibody (SR1751) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21632
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR1751 expressed in HEK293
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human MEK1/2 (Uniprot #: Q02750, P36507)
Specificity
Detects endogenous levels of total MEK1/2
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit MEK1/2 Antibody (SR1751) - BSA Free (NBP3-21632) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MEK1/2 Antibody (SR1751) - BSA Free
Western Blot: MEK1/2 Antibody (SR1751) [NBP3-21632] -
Western Blot: MEK1/2 Antibody (SR1751) [NBP3-21632] - Western blot analysis using NBP3-21632 in (1) 293T cell lysate; (2) A549 cell lysate.
Immunohistochemistry: MEK1/2 Antibody (SR1751) [NBP3-21632] -
Immunohistochemistry: MEK1/2 Antibody (SR1751) [NBP3-21632] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21632.
Immunocytochemistry/Immunofluorescence: MEK1/2 Antibody (SR1751) [NBP3-21632] -
Immunocytochemistry/Immunofluorescence: MEK1/2 Antibody (SR1751) [NBP3-21632] - Immunofluorescent analysis of Hela cells, using NBP3-21632.Applications for MEK1/2 Antibody (SR1751) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:100-1:500
Immunoprecipitation
1:50-1:100
Western Blot
1:5000-1:10000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MEK1/MEK2
MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Symbol
MAP2K1
Additional MEK1/MEK2 Products
Product Documents for MEK1/2 Antibody (SR1751) - BSA Free
Product Specific Notices for MEK1/2 Antibody (SR1751) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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