MDM2/HDM2 Antibody (SMP14) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2736APC
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SMP14
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]
Epitope
Amino acids 154 - 167.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 15625077). Mouse reactivity reported in scientific literature (PMID: 25981963).
Localization
Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm).
Specificity
The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for MDM2/HDM2 Antibody (SMP14) [Allophycocyanin]
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry - Frozen and Paraffin, Immunoprecipitation and Western blot. This antibody has also been reported useful in IHC on resin tissue sections. Staining of formalin-fixed paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Recommended applications are based on validated applications from the unconjugated base product NB100-2736. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Recommended applications are based on validated applications from the unconjugated base product NB100-2736. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: MDM2/HDM2
Long Name
Double Minute 2 Protein
Alternate Names
HDM2
Gene Symbol
MDM2
Additional MDM2/HDM2 Products
Product Documents for MDM2/HDM2 Antibody (SMP14) [Allophycocyanin]
Product Specific Notices for MDM2/HDM2 Antibody (SMP14) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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