MDA5 Antibody (SR1076) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21840
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR1076 expressed in HEK293
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human MDA5 (Uniprot #: Q9BYX4)
Specificity
Detects endogenous levels of total MDA5
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit MDA5 Antibody (SR1076) - BSA Free (NBP3-21840) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MDA5 Antibody (SR1076) - BSA Free
Western Blot Shows MDA5 Specificity Using knockdown Cell Line.
Western blot shows lysates of DMS 53 parental cell line and MDA5 knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with MDA5 Antibody (SR1076) (Catalog # NBP3-21840) followed by HRP-conjugated secondary antibody. A specific band was detected for MDA5 at approximately 116.6 kDa (as indicated) in the parental DMS 53 cell line, but is not detectable in knockdown DMS 53 cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] -
Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] - Western blot analysis using NBP3-21840 in THP1 cell lysate.Applications for MDA5 Antibody (SR1076) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Knockdown Validated
Knockdown Validated from YCharOS Inc. (YCharOS.com)
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IFIH1/MDA5
Long Name
Melanoma Differentiation-Associated Protein 5
Alternate Names
AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1
Gene Symbol
IFIH1
Additional IFIH1/MDA5 Products
Product Documents for MDA5 Antibody (SR1076) - BSA Free
Product Specific Notices for MDA5 Antibody (SR1076) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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