MCM7 Antibody (S07-7A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14878

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14878-100ul
NBP3-14878-25ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-7A4 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MCM7 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human MCM7 (Uniprot#: P33993)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit MCM7 Antibody (S07-7A4) (NBP3-14878) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MCM7 Antibody (S07-7A4)

Western Blot: MCM7 Antibody (S07-7A4) [NBP3-14878]

Western Blot: MCM7 Antibody (S07-7A4) [NBP3-14878]

Western Blot: MCM7 Antibody (S07-7A4) [NBP3-14878] - Western blot detection of MCM7 in K562, C6, 3T3, Hela cell lysates using MCM7 Rabbit mAb (1:1000 diluted). Predicted band size: 81kDa. Observed band size: 81kDa.

Applications for MCM7 Antibody (S07-7A4)

Application
Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM7

The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).

Long Name

Minichromosome Maintenance Protein 7

Alternate Names

CDC47, MCM2, P1CDC47, P85MCM, PNAS146, PPP1R104, Protein Phosphatase 1

Gene Symbol

MCM7

Additional MCM7 Products

Product Documents for MCM7 Antibody (S07-7A4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MCM7 Antibody (S07-7A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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