MCM4 Antibody (23GB2495)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50262

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NBP3-50262-100ul
NBP3-50262-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2495

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MCM4 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human MCM4 (Uniprot: P33991)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM4 Antibody (23GB2495)

MCM4 Antibody (23GB2495)

Immunocytochemistry/ Immunofluorescence: MCM4 Antibody (23GB2495)[NBP3-50262] -

Immunocytochemical staining of HepG2 cells with MCM4 antibody (1:1,000). Nuclei were stained blue with DAPI; MCM4 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Applications for MCM4 Antibody (23GB2495)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCM4

MCM4 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Long Name

minichromosome maintenance complex component 4

Alternate Names

CDC21, CDC54, hCdc21, IMD54, NKCD, NKGCD, P1-CDC21

Gene Symbol

MCM4

Additional MCM4 Products

Product Documents for MCM4 Antibody (23GB2495)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MCM4 Antibody (23GB2495)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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