Max Antibody (103) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90153
Recombinant Monoclonal Antibody
![Western Blot: Max Antibody (103) [NBP2-90153]](https://resources.bio-techne.com/images/products/Max-Antibody-103-Western-Blot-NBP2-90153-img0004.jpg "Western Blot: Max Antibody (103) [NBP2-90153]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 103 expressed in HEK293
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Max (Accession#: NP_002373; Met1-Ser160).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Max Antibody (103) - Azide and BSA Free
Western Blot: Max Antibody (103) [NBP2-90153]
Western Blot: Max Antibody (103) [NBP2-90153] - Lane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:20 kDa(We are unsure as to the identity of these extra bands.)![Flow Cytometry: Max Antibody (103) [NBP2-90153]](https://resources.bio-techne.com/images/products/Max-Antibody-103-Flow-Cytometry-NBP2-90153-img0002.jpg "Flow Cytometry: Max Antibody (103) [NBP2-90153]")
Flow Cytometry: Max Antibody (103) [NBP2-90153]
Flow Cytometry: Max Antibody (103) [NBP2-90153] - Analysis of Human MAX expression on Raji cells stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.![Immunoprecipitation: Max Antibody (103) [NBP2-90153]](https://resources.bio-techne.com/images/products/Max-Antibody-103-Immunoprecipitation-NBP2-90153-img0003.jpg "Immunoprecipitation: Max Antibody (103) [NBP2-90153]")
Immunoprecipitation: Max Antibody (103) [NBP2-90153]
Immunoprecipitation: Max Antibody (103) [NBP2-90153] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-MAX rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAX rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDaApplications for Max Antibody (103) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:25000-1:50000
Flow Cytometry
1:100-1:500
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Max
Long Name
Myc associated factor X
Alternate Names
BHLHD4, bHLHd4MGC11225, bHLHd5, bHLHd6, bHLHd7, bHLHd8, Class D basic helix-loop-helix protein 4, helix-loop-helix zipper protein, MAX protein, MGC10775, MGC18164, MGC34679, MGC36767, MYC associated factor X, Myc-associated factor X, orf1, protein max
Gene Symbol
MAX
Additional Max Products
Product Documents for Max Antibody (103) - Azide and BSA Free
Product Specific Notices for Max Antibody (103) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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