MASTL Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-22987
![Western Blot: MASTL Antibody [NBP1-22987]](https://resources.bio-techne.com/images/products/MASTL-Antibody-Western-Blot-NBP1-22987-img0009.jpg "Western Blot: MASTL Antibody [NBP1-22987]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human microtubule associated serine/threonine kinase-like using the numbering given in entry NP_116233.2 (GeneID 84930).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MASTL Antibody - BSA Free (NBP1-22987) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-MASTL Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MASTL Antibody - BSA Free
Western Blot: MASTL Antibody [NBP1-22987]
Western Blot: MASTL Antibody [NBP1-22987] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987]](https://resources.bio-techne.com/images/products/MASTL-Antibody-Immunohistochemistry-Paraffin-NBP1-22987-img0008.jpg "Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987]")
Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987]
Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MASTL used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB![Immunoprecipitation: MASTL Antibody [NBP1-22987]](https://resources.bio-techne.com/images/products/MASTL-Antibody-Immunoprecipitation-NBP1-22987-img0010.jpg "Immunoprecipitation: MASTL Antibody [NBP1-22987]")
Immunoprecipitation: MASTL Antibody [NBP1-22987]
Immunoprecipitation: MASTL Antibody [NBP1-22987] - Detection of human MASTL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MASTL antibody NBP1-22987 (lot NBP1-22987-4) used for IP at 3 ug per reaction. MASTL was also immunoprecipitated by a previous lot of this antibody (lot NBP1-22987-3). For blotting immunoprecipitated MASTL, NBP1-22987 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for MASTL Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MASTL
Long Name
MASTL
Alternate Names
Greatwall, THC2
Entrez Gene IDs
84930 (Human)
Gene Symbol
MASTL
UniProt
Additional MASTL Products
Product Documents for MASTL Antibody - BSA Free
Product Specific Notices for MASTL Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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