Malondialdehyde Antibody (6H6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59366
![Western Blot: Malondialdehyde Antibody (6H6) [NBP2-59366]](https://resources.bio-techne.com/images/products/Malondialdehyde-Antibody-6H6-Western-Blot-NBP2-59366-img0006.jpg "Western Blot: Malondialdehyde Antibody (6H6) [NBP2-59366]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Non-species specific
Applications
Validated:
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6H6
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Malondialdehyde Antibody (6H6) - BSA Free (NBP2-59366) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Malondialdehyde Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Malondialdehyde Antibody (6H6) - BSA Free
Western Blot: Malondialdehyde Antibody (6H6) [NBP2-59366]
Western Blot: Malondialdehyde Antibody (6H6) [NBP2-59366] - Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6 (NBP2-59366). Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 ug). Lane 3: Malondialdehyde-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug) . Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59366) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.![Immunocytochemistry/ Immunofluorescence: Malondialdehyde Antibody (6H6) [NBP2-59366]](https://resources.bio-techne.com/images/products/Malondialdehyde-Antibody-6H6-Immunocytochemistry-Immunofluorescence-NBP2-59366-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Malondialdehyde Antibody (6H6) [NBP2-59366]")
Immunocytochemistry/ Immunofluorescence: Malondialdehyde Antibody (6H6) [NBP2-59366]
Immunocytochemistry/Immunofluorescence: Malondialdehyde Antibody (6H6) [NBP2-59366] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.![Flow Cytometry: Malondialdehyde Antibody (6H6) [NBP2-59366]](https://resources.bio-techne.com/images/products/Malondialdehyde-Antibody-6H6-Flow-Cytometry-NBP2-59366-img0007.jpg "Flow Cytometry: Malondialdehyde Antibody (6H6) [NBP2-59366]")
Flow Cytometry: Malondialdehyde Antibody (6H6) [NBP2-59366]
Flow Cytometry: Malondialdehyde Antibody (6H6) [NBP2-59366] - Flow Cytometry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6 (NBP2-59366). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59366) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.Applications for Malondialdehyde Antibody (6H6) - BSA Free
Application
Recommended Usage
ELISA
1:1000
Flow Cytometry
1:50
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Malondialdehyde
Alternate Names
3-Propanedial;Malonaldehyde;Malondialdehyde;MDA
Additional Malondialdehyde Products
Product Documents for Malondialdehyde Antibody (6H6) - BSA Free
Product Specific Notices for Malondialdehyde Antibody (6H6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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