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Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [DyLight 488]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97310G

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NBP1-97310G
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human JARID1B protein (between residues 800-850) [UniProt Q9UGL1]

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP1-97310. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

Jumonji AT-rich interactive domain 1B (JARID1B) also known as Jumonji/ARID domain-containing protein 1B, Lysine-specific demethylase 5B (KDM5B), Cancer/testis antigen 31 (CT31), Histone demethylase JARID1B, PLU-1, and Retinoblastoma-binding protein 2 homolog 1 (RBP2-H1), is a member of the JARID 1 family. The JARID 1 proteins (JARID1A, JARID1B, JARID1C and JARID1D) catalyze the demethylation of histones on lysine, and JARID1B specifically demethylates lysine 4 on Histone H3. JARID1B functions as a transcriptional co-repressor for FOXG1B and PAX9. In addition, JARID1B may play a considerable role in cancer research as it assists in the propagation of breast cancer cells by repressing the tumor suppressor genes BRCA1 and HOXA5. Conversely, JARID1B acts as a direct suppressor of melanoma. As result, the JARID1B protein is up regulated in breast cancer and down regulated in melanoma and glioblastomas. In general, JARID1B expression is ubiquitous; with the highest expression seen in testis. JARID1B is subject to post translational phosphorylation and has two known isoforms; isoform 1: 175.6kDa and isoform 2: 179.4kDa.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Gene Symbol

KDM5B

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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