Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-96416
![Immunocytochemistry/ Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416]](https://resources.bio-techne.com/images/products/Lymphatic-Endothelial-Cells-Antibody-LA102-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-96416-img0015.jpg "Immunocytochemistry/ Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # LA102
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Collagenase- and neuraminidase-treated mouse benign lymphangiomas
Specificity
LA102 reacts with mouse lymphatic vessels except the thoracic duct and the marginal sinus of lymph nodes, but not with any blood vessels. LA102 recognized a protein of around 26 kDa.The antigen recognized by LA102 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides lymphatics, some lymphoid cells are also recognized.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416]
Immunocytochemistry/Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416] - Immunofluorescence staining of mouse Lymphatic Endothelial Cells antigen in primary mouse endothelial cells (mEC) using a monoclonal antibody directed against collagenase- and neuraminidase-treated mouse benign lymphangiomas. As secondary antibody goat anti-rat ALEXA Flour 488 (1:600) was used.![Immunocytochemistry/ Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416]](https://resources.bio-techne.com/images/products/Lymphatic-Endothelial-Cells-Antibody-LA102-Immunocytochemistry-Immunofluorescence-NB110-96416-img0013.jpg "Immunocytochemistry/ Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416]")
Immunocytochemistry/ Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416]
Immunocytochemistry/Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416] - Lymphatic Endothelial Cells Antibody (LA102) [NB110-96416] - Immunofluorescence staining in primary mouse endothelial cells (mEC) with Lymphatic Endothelial Cells Antibody (LA102) and counter staining of nuclei with Dapi. As secondary antibody goat anti-rat ALEXA Flour 488 was used 1:400.Applications for Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
2-5 ug/ml
Application Notes
IHC-Fr: acetone fixed or 4% paraformaldehyde in PBS for less than 10 min.
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lymphatic Endothelial Cells
Alternate Names
LEC26
Additional Lymphatic Endothelial Cells Products
Product Documents for Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free
Product Specific Notices for Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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