LRH-1/NR5A2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27196
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Chinese Hamster, Equine, Guinea Pig, Opossum, Primate, Rabbit, Rhesus Macaque, Zebrafish
Cited:
Porcine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated
Cited:
Western Blot, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 140-190 from human NR5A2 was used as the immunogen for this antibody.
Reactivity Notes
Orangutan (100%), Chimpanzee (100%), New World Monkey (100%), Panda (100%), Great Ape (100%), Mole Rat (100%), White cheeked gibbon (100%), Platypus (100%), Zebra finch (100%), Catfish (100%), Frog (100%), Carolina anole (100%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LRH-1/NR5A2 Antibody - BSA Free
Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196]
Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2 in HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH3T3 and 4) RAW lysate probed with 3 ug/ml of NR5A2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196]
Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2/LRH1 in human pancreas tissue using NR5A2/LRH1 antibody at 5 ug/ml.Western Blot: LRH-1/NR5A2 Antibody - BSA Free [NBP2-27196] -
Effects of NR5A2 knockdown on progesterone biosynthesis. (A) gene expression of NR5A2 after siRNA transfection; (B) effects of NR5A2 knockdown on progesterone secretion; (C) gene expression of key factors (StAR, Cyp11a1, HSD3B, and NPC1) involved in progesterone biosynthesis; (D,E) protein expression of NR5A2, StAR, Cyp11a1, and HSD3B. Primary pGCs were transfected with NR5A2 siRNA (siNR5A2) or scramble siRNA (siNC). Six hours later, the medium was changed to fresh medium with DMSO or DAPT, followed by further culture for 48 h. * means significant difference at p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31978970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for LRH-1/NR5A2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
5ug/ml
Knockdown Validated
reported in scientific literature (PMID 31978970)
Western Blot
1-5ug/ml~
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRH-1/NR5A2
Long Name
Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2
Alternate Names
B1F2, FTZ-F1 beta, LRH1, NR5A2
Gene Symbol
NR5A2
Additional LRH-1/NR5A2 Products
Product Documents for LRH-1/NR5A2 Antibody - BSA Free
Product Specific Notices for LRH-1/NR5A2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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