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LMAN1 Antibody (1H6) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27953CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse monoclonal LMAN1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human LMAN1.

Specificity

At least two isoforms of LMAN1 are known to exist.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for LMAN1 Antibody (1H6) [CoraFluor™ 1]

LMAN1 Antibody (1H6) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for LMAN1 Antibody (1H6) [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: LMAN1

Protein trafficking along the endoplasmic reticulum (ER) to the Golgi apparatus occurs by vesicular transport between membranous compartments. LMAN1 is a mannose-specific lectin transport receptor protein which cycles between the ER and the Golgi complex. LMAN1 is involved in intracellular transport of many glycoproteins. LMAN1 is useful in studying mechanisms underlying protein trafficking early in the secretory pathway.

Alternate Names

ERGIC-53, ERGIC53ER-Golgi intermediate compartment 53 kDa protein, F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53, FMFD1, Gp58, intracellular mannose specific lectin, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, lectin, mannose-binding, 1, MCFD1coagulation factor V-factor VIII combined deficiency, MR60, protein ERGIC-53

Gene Symbol

LMAN1

Additional LMAN1 Products

Product Documents for LMAN1 Antibody (1H6) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LMAN1 Antibody (1H6) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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