LIN-28B Antibody (7D7F7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16815
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7D7F7 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human LIN-28B (Q6ZN17). MAEGGASKGGGEEPGKLPEPAEEESQVLRGTGHCKWFNVRMGFGFISMINREGSPLDIPVDVFVHQSKLFMEGFRSLKEGEPVEFTFKKSSKGLESIRVTGPGGSPCLGSERRPKGKTLQKRKPKGDRCYNCGGLDHHAKECSLPPQPKKCHYCQSIMHMVAN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit LIN-28B Antibody (7D7F7) (NBP3-16815) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for LIN-28B Antibody (7D7F7)
![Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815]](https://resources.bio-techne.com/images/products/LIN-28B-Antibody-7D7F7-Western-Blot-NBP3-16815-img0002.jpg "Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815]")
Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815]
Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.![Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815]](https://resources.bio-techne.com/images/products/LIN-28B-Antibody-7D7F7-Immunohistochemistry-Paraffin-NBP3-16815-img0001.jpg "Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815]")
Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815]
Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry of paraffin-embedded human placenta using LIN-28B Rabbit mAb (NBP3-16815) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] -
Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.Applications for LIN-28B Antibody (7D7F7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: LIN-28B
Long Name
LIN-28 Homolog B
Alternate Names
CSDD2, Lin-28.2, LIN28B
Gene Symbol
LIN28B
Additional LIN-28B Products
Product Documents for LIN-28B Antibody (7D7F7)
Product Specific Notices for LIN-28B Antibody (7D7F7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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