Leptin R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-40796

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NB100-40796
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Control

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

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Product Specifications

Immunogen

Peptide with sequence C-TQDDIEKHQSDAG corresponding to internal region according to NP_002294.2, NP_001003679.1, NP_001003680.1.

Specificity

This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Leptin R Antibody

Western Blot: Leptin R Antibody [NB100-40796]

Western Blot: Leptin R Antibody [NB100-40796]

Western Blot: Leptin R Antibody [NB100-40796] - (1.75ug/ml) staining of Human Cerebellum (A), and (1.5ug/ml) Mouse Foetal Brain (B) and Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry: Leptin R Antibody [NB100-40796]

Immunohistochemistry: Leptin R Antibody [NB100-40796]

Immunohistochemistry: Leptin R Antibody [NB100-40796] - (6ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
Flow Cytometry: Leptin R Antibody [NB100-40796]

Flow Cytometry: Leptin R Antibody [NB100-40796]

Flow Cytometry: Leptin R Antibody [NB100-40796] - Analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for Leptin R Antibody

Application
Recommended Usage

Flow Cytometry

10ug/ml

Immunohistochemistry

4-6ug/ml

Immunohistochemistry-Paraffin

4-6ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

1-2ug/ml
Application Notes
Western blot: Approx 140-150kDa band observed in Human Cerebellum, Mouse Foetal Brain and Rat Brain lysates (calculated MW of 132kDa according to Human NP_002294.2 and 130.8kDa according to Mouse NP_666258.2 and Rat NP_036728.1). This molecular weight is routinely observed by other sources. Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Leptin R

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Long Name

Leptin Receptor

Alternate Names

B219, CD295, LEPR, LeptinR, OB R

Entrez Gene IDs

3953 (Human)

Gene Symbol

LEPR

UniProt

NP_002294.2 (Human)

Additional Leptin R Products

Product Documents for Leptin R Antibody

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Certificate of Analysis

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Product Specific Notices for Leptin R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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