Legumain/Asparaginyl Endopeptidase Antibody (6X5U6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16645

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16645-20ul
NBP3-16645-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6X5U6 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Legumain/Asparaginyl Endopeptidase Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 210-302 of human Legumain/Asparaginyl Endopeptidase (Q99538). ANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) (NBP3-16645) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Legumain/Asparaginyl Endopeptidase Antibody (6X5U6)

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [NBP3-16645]

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [NBP3-16645]

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [NBP3-16645] - Western blot analysis of extracts of various cell lines, using Legumain/Asparaginyl Endopeptidase (LGMN) Rabbit mAb (NBP3-16645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Legumain/Asparaginyl Endopeptidase Antibody (6X5U6)

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [NBP3-16645] -

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [NBP3-16645] - Western blot analysis of various lysates using Legumain/Asparaginyl Endopeptidase Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Applications for Legumain/Asparaginyl Endopeptidase Antibody (6X5U6)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Legumain/Asparaginyl Endopeptidase

Legumain encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq]

Alternate Names

AEP, Asparaginyl Endopeptidase, LGMN

Gene Symbol

LGMN

Additional Legumain/Asparaginyl Endopeptidase Products

Product Documents for Legumain/Asparaginyl Endopeptidase Antibody (6X5U6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Legumain/Asparaginyl Endopeptidase Antibody (6X5U6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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