Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Janelia Fluor 525

Antibody Source

Monoclonal Mouse IgG2B Clone # 755103

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lck Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human Lck Y394 site
Accession # P06239

Modification

p Tyr394

Specificity

Detects human Lck when phosphorylated at Y394. Due to high sequence homology, this antibody may cross-react with phosphorylated members of the Src family.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Lck [p Tyr394] Antibody (755103) [Janelia Fluor® 525]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lck

The human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4).

Long Name

Lymphocyte Protein Tyrosine Kinase

Alternate Names

Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16

Gene Symbol

LCK

Additional Lck Products

Product Documents for Lck [p Tyr394] Antibody (755103) [Janelia Fluor® 525]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lck [p Tyr394] Antibody (755103) [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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