KLF9 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88507

Novus Biologicals, part of Bio-Techne
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NBP1-88507
NBP1-88507-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KLF9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:QCLVSISNRAAVPEHGVAPDAERLRLPEREVTKEHGDPGDTWKDYCTLVTIAKSLLDLNKYRPIQTPSVCSDSLESPDEDMGSDSDVTTESGSSPSHSPEERQDPGSAPSPLSLLHPGVAAKGKHASEKRHKCPYS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit KLF9 Antibody - BSA Free (NBP1-88507) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for KLF9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: KLF9 Antibody [NBP1-88507]

Immunocytochemistry/ Immunofluorescence: KLF9 Antibody [NBP1-88507]

Immunocytochemistry/Immunofluorescence: KLF9 Antibody [NBP1-88507] - Staining of human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Antibody staining is whown in green.
KLF9 Antibody - BSA Free Western Blot: KLF9 Antibody - BSA Free [NBP1-88507]

Western Blot: KLF9 Antibody - BSA Free [NBP1-88507]

Analysis in control (vector only transfected HEK293T lysate) and KLF9 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
KLF9 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: KLF9 Antibody - BSA Free [NBP1-88507]

Chromatin Immunoprecipitation-exo-Seq: KLF9 Antibody - BSA Free [NBP1-88507]

ChIP-Exo-Seq composite graph for Anti-KLF9 (NBP1-88507) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for KLF9 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF9

KLF9 is a gene that codes for a transcription factor that activates mRNA synthesis on a selective basis from genes that only contain tandem repeats of GC boxes, otherwise the gene will be suppressed, and it has a length of 244 amino acids, and amass of approximately 27 kDa. Studies have been conducted on diseases and disorders relating to this gene, including Kabuki syndrome, otosclerosis, leiomyoma, colorectal cancer, carcinoma, glioblastoma, cholesterol, thyroiditis, and leukemia. KLF9 has also been shown to have interactions with PGR, SP1, SIN3A, HDAC7, and ELAV1 in pathways such as the diurnally regulated gene pathway.

Alternate Names

basic transcription element binding protein 1, Basic transcription element-binding protein 1, BTEB1GC-box-binding protein 1, BTEBBTE-binding protein 1, Krueppel-like factor 9, Kruppel-like factor 9, Transcription factor BTEB1

Gene Symbol

KLF9

Additional KLF9 Products

Product Documents for KLF9 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KLF9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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