KLF4 Antibody (CL5785) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61427
![Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427]](https://resources.bio-techne.com/images/products/KLF4-Antibody-CL5785-Immunocytochemistry-Immunofluorescence-NBP2-61427-img0001.jpg "Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427]")
![Western Blot: KLF4 Antibody (CL5785) [NBP2-61427]](https://resources.bio-techne.com/images/products/KLF4-Antibody-CL5785-Western-Blot-NBP2-61427-img0008.jpg "Western Blot: KLF4 Antibody (CL5785) [NBP2-61427]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL5785
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from O43474, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Mouse, Rat (89%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for KLF4 Antibody (CL5785) - BSA Free
Western Blot: KLF4 Antibody (CL5785) [NBP2-61427]
Western Blot: KLF4 Antibody (CL5785) [NBP2-61427] - Analysis in human cell line U-2197.![Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427]](https://resources.bio-techne.com/images/products/KLF4-Antibody-CL5785-Immunocytochemistry-Immunofluorescence-NBP2-61427-img0002.jpg "Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427]")
Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427]
Immunocytochemistry/Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of U-2197cells using the Anti-KLF4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]](https://resources.bio-techne.com/images/products/KLF4-Antibody-CL5785-Immunohistochemistry-Paraffin-NBP2-61427-img0006.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]")
Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]
Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]](https://resources.bio-techne.com/images/products/KLF4-Antibody-CL5785-Immunohistochemistry-Paraffin-NBP2-61427-img0003.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]")
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]](https://resources.bio-techne.com/images/products/KLF4-Antibody-CL5785-Immunohistochemistry-Paraffin-NBP2-61427-img0004.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]")
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]](https://resources.bio-techne.com/images/products/KLF4-Antibody-CL5785-Immunohistochemistry-Paraffin-NBP2-61427-img0005.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]")
Applications for KLF4 Antibody (CL5785) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KLF4
Long Name
Kruppel-Like Factor 4
Alternate Names
EZF
Gene Symbol
KLF4
Additional KLF4 Products
Product Documents for KLF4 Antibody (CL5785) - BSA Free
Product Specific Notices for KLF4 Antibody (CL5785) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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