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KIF2C Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2588

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NB100-2588
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_006836.1 (GeneID 11004).

Reactivity Notes

Mouse reactivity reported in (PMID: 23055517).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KIF2C Antibody - BSA Free

Western Blot: KIF2C Antibody [NB100-2588]

Western Blot: KIF2C Antibody [NB100-2588]

Western Blot: KIF2C Antibody [NB100-2588] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAK antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds..
Immunoprecipitation: KIF2C Antibody [NB100-2588]

Immunoprecipitation: KIF2C Antibody [NB100-2588]

Immunoprecipitation: KIF2C Antibody [NB100-2588] - Detection of human MCAK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCAK antibody NB100-2588 used for IP at 3 ug per reaction. MCAK was also immunoprecipitated by rabbit anti-MCAK antibody NB100-2587. For blotting immunoprecipitated MCAK, NB100-2588 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for KIF2C Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KIF2C

The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation.

Alternate Names

kinesin family member 2C, kinesin-like 6 (mitotic centromere-associated kinesin), Kinesin-like protein 6, kinesin-like protein KIF2C, MCAKKNSL6kinesin-like 6, Mitotic centromere-associated kinesin

Entrez Gene IDs

11004 (Human)

Gene Symbol

KIF2C

UniProt

Additional KIF2C Products

Product Documents for KIF2C Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for KIF2C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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