CD11b Antibody (3A33)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-27874

Discontinued Product
NBP1-27874 has been discontinued. View all CD11b/Integrin alpha M products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 3A33

Concentration

0.5 mg/ml

View all CD11b/Integrin alpha M Antibodies »

Product Specifications

Immunogen

Rat Monoclonal CD11b Antibody (3A33) was generated by immunizing host with peritoneal macrophages from B6D2 hybrid mice.

Reactivity Notes

Mouse.

Specificity

alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer

Marker

Microglia Marker, Macrophage Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for CD11b Antibody (3A33)

Application
Recommended Usage

Flow Cytometry

<= 1 ug/10^6 cells

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD11b/Integrin alpha M

CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). The CD11b/CD18 (Mac-1) heterodimer has a theoretical molecular weight of 127kDa but is often observed near 170kDa due to post translational modifications. Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix. Integrins have diverse roles in several biological processes, including cell migration during development, wound healing, cell differentiation, and apoptosis. The particular integrin CD11b is known as a pro-inflammatory molecule given its ability to promote phagocyte cytotoxic functions while enhancing several effector molecules such as FcGR, uPAR, and CD14 (1). In macrophages, CpG-DNA stimulates lysosomal degradation and vacuolar acidification, subsequently promoting CD11b release via TLR9 (2). During an acute infection, high CD11b/Mac-1 expression on antigen-specific CD8 (+) T cells can signify recent activation of the immune system, whereas naive T cells and virus-specific memory CD8 (+) T cells express little or no Mac-1 when exposed to a virus (3). Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder (4).

References

1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373

2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677

3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593

4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71

Alternate Names

CD11b, Integrin alpha M, ITGAM

Gene Symbol

ITGAM

Additional CD11b/Integrin alpha M Products

Product Documents for CD11b Antibody (3A33)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD11b Antibody (3A33)

Inquire about low endotoxin, azide-free CD11b antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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