Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43283B

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # DATK32

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Integrin alpha 4 beta 7/LPAM-1 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was LPAM1.

Epitope

The monoclonal antibody DATK32 recognizes a conformational epitope on the heterodimer, integrin alpha 4 beta 7 (LPAM-1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) [Biotin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This DATK32 antibody has been tested tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha 4 beta 7/LPAM-1

The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha4beta7), which is a heterodimer of the the 154 kDa alpha4 subunit and the 130 kDa beta7 subunit. In mouse, the beta7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.

Alternate Names

CD49d/beta7, LPAM-1, LPAM1

Gene Symbol

ITGA4

Additional Integrin alpha 4 beta 7/LPAM-1 Products

Product Documents for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) [Biotin]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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