Insulin B chain Antibody

Immunohistochemistry: Insulin B chain Antibody [NBP3-33144]

Immunohistochemistry: Insulin B chain Antibody [NBP3-33144] - Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Insulin B chain antibody (NBP3-33144) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Insulin B chain Antibody [NBP3-33144]

Flow Cytometry: Insulin B chain Antibody [NBP3-33144] - Flow cytometric analysis of Insulin B chain was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-33144, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).