INO80 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78759
![Western Blot: INO80 Antibody [NBP1-78759]](https://resources.bio-techne.com/images/products/INO80-Antibody-Western-Blot-NBP1-78759-img0003.jpg "Western Blot: INO80 Antibody [NBP1-78759]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1506 and 1556 of human INO80 homolog using the numbering given in entry NP_060023.1 (GeneID 54617).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit INO80 Antibody - BSA Free (NBP1-78759) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for INO80 Antibody - BSA Free
Western Blot: INO80 Antibody [NBP1-78759]
Western Blot: INO80 Antibody [NBP1-78759] - Detection of human INO80 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, A-549, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INO80 antibody NBP1-78759 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.![Western Blot: INO80 Antibody [NBP1-78759]](https://resources.bio-techne.com/images/products/INO80-Antibody-Western-Blot-NBP1-78759-img0002.jpg "Western Blot: INO80 Antibody [NBP1-78759]")
Western Blot: INO80 Antibody [NBP1-78759]
Western Blot: INO80 Antibody [NBP1-78759] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-INO80 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. INO80 was also immunoprecipitated by rabbit anti-INO80 antibody NBP1-78758 which recognizes an upstream epitope.![Immunoprecipitation: INO80 Antibody [NBP1-78759]](https://resources.bio-techne.com/images/products/INO80-Antibody-Immunoprecipitation-NBP1-78759-img0004.jpg "Immunoprecipitation: INO80 Antibody [NBP1-78759]")
Immunoprecipitation: INO80 Antibody [NBP1-78759]
Immunoprecipitation: INO80 Antibody [NBP1-78759] - Detection of human INO80 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INO80 antibody lot NBP1-78759-2 used for IP at 6 ug per reaction. INO80 was also immunoprecipitated by a previous lot of this antibody NBP1-78759-1 and rabbit anti-INO80 antibody NBP1-78758-2. For blotting immunoprecipitated INO80, A303-371A was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for INO80 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 mcg/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: INO80
Alternate Names
EC 3.6.1, EC 3.6.1.23, EC 3.6.4.12, hINO80INO80 complex subunit A, homolog of yeast INO80, Ino80, INO80 complex homolog 1 (S. cerevisiae), INO80 homolog (S. cerevisiae), INO80A, INOC1putative DNA helicase INO80 complex homolog 1, KIAA1259
Gene Symbol
INO80
UniProt
Additional INO80 Products
Product Documents for INO80 Antibody - BSA Free
Product Specific Notices for INO80 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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