Influenza A nucleoprotein Antibody (HL1098) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13717

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-13717
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1098 expressed in HEK293

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Influenza A nucleoprotein Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A nucleoprotein (A/California/7/2009). The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Influenza A nucleoprotein Antibody (HL1098) - Azide and BSA Free

Western Blot: Influenza A nucleoprotein Antibody (HL1098) [NBP3-13717]

Western Blot: Influenza A nucleoprotein Antibody (HL1098) [NBP3-13717]

Western Blot: Influenza A nucleoprotein Antibody (HL1098) [NBP3-13717] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody [HL1098] (NBP3-13717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Influenza A nucleoprotein Antibody (HL1098) [NBP3-13717]

Immunocytochemistry/ Immunofluorescence: Influenza A nucleoprotein Antibody (HL1098) [NBP3-13717]

Immunocytochemistry/Immunofluorescence: Influenza A nucleoprotein Antibody (HL1098) [NBP3-13717] - Immunofluorescent analysis of influenza virus infected cells using Influenza A nucleoprotein antibody (NBP3-13717). Sample: Influenza A and B Virus infected cells slide. Green: Influenza A nucleoprotein antibody (NBP3-13717) diluted at 1:100.
Sandwich ELISA: Influenza A nucleoprotein Antibody (HL1098) [NBP3-13717] - Sandwich ELISA detection of recombinant Influenza A nucleoprotein protein (A/California/2009 (H1N1)), DDDDK Tag using antibodies as below. Capture: Influenza A nucleoprotein antibody [HL1098] (NBP3-13717) (5 ug/mL) Detection: HRP-conjugated Influenza A nucleoprotein antibody [HL1103] (NBP3-13721) (1 ug/mL) Please notice that NBP3-13721 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13717. Please contact us for custom HRP-conjugated antibody.
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Applications for Influenza A nucleoprotein Antibody (HL1098) - Azide and BSA Free

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Sandwich ELISA

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza A nucleoprotein

The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

Alternate Names

Influenza A virus NP, NP, NP-A, Nucleocapsid protein, Nucleoprotein

Additional Influenza A nucleoprotein Products

Product Documents for Influenza A nucleoprotein Antibody (HL1098) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Influenza A nucleoprotein Antibody (HL1098) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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