IL6R Antibody (DM152)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48288
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # DM152
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
IL6R recombinant protein expressed in mammalian cells (Accession# P08887). The exact amino acid sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).
Scientific Data Images for IL6R Antibody (DM152)
Flow Cytometry: IL6R Antibody (DM152) [NBP3-48288]
Flow cytometry analysis with Anti-IL6R (DM152) on HEK293 cells transfected with human IL6R (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
ELISA: IL6R Antibody (DM152) [NBP3-48288]
ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human IL6R protein, His tagged protein PME100109 can bind Rabbit anti-IL6R monoclonal antibody (clone: DM152)in a linear range of 1-100 ng/ml.
Flow Cytometry: IL6R Antibody (DM152) [NBP3-48288]
Flow cytometry analysis of antigen binding of rabbit anti-human IL6R mAb(NBP3-48288). (A) NBP3-48288 does not bind to CHO-S cells that do not express IL6R. (B) A clear peak shift of NBP3-48288 was seen compared to the control when incubated with IL6R-expressing THP-1 cells, indicating strong binding of NBP3-48288 to IL6R. Antibodies were incubated at 5 ug/mL.Applications for IL6R Antibody (DM152)
Application
Recommended Usage
ELISA
1:5000-10000
Flow Cytometry
1:100
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified from cell culture supernatant
Reconstitution
Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.
Formulation
PBS, pH 7.4, 5-8% Trehalose
Preservative
Trehalose
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Background: IL6R
Alternate Names
CD126, CD126 antigen, gp80, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-1, IL6RA, IL-6RA, IL-6R-alpha, interleukin 6 receptor, interleukin 6 receptor alpha subunit, interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, MGC104991
Gene Symbol
IL6R
Additional IL6R Products
Product Specific Notices for IL6R Antibody (DM152)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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