IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71834
![Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4)Azide and BSA Free [NBP2-71834]](https://resources.bio-techne.com/images/products/IL-36-alpha-IL-1F6-Antibody-OTI1A4-Azide-and-BSA-Free-Western-Blot-NBP2-71834-img0001.jpg "Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4)Azide and BSA Free [NBP2-71834]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1A4
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human IL1F6 (NP_055255) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free
Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4)Azide and BSA Free [NBP2-71834]
Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.![Immunocytochemistry/ Immunofluorescence: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834]](https://resources.bio-techne.com/images/products/IL-36-alpha-IL-1F6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71834-img0003.jpg "Immunocytochemistry/ Immunofluorescence: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834]")
Immunocytochemistry/ Immunofluorescence: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834]
Immunocytochemistry/Immunofluorescence: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6.
CyTOF-ready: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IL1F6 antibody, and then analyzed by flow cytometry.
Applications for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IL-36 alpha/IL-1F6
Interleukin 36 alpha (IL-36 alpha), also known as IL-1F6 and FIL1 epsilon, is a member of the IL-1 family of proteins. Cells reported to express IL-36 alpha include monocytes, B cells, and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Recombinant IL-36 alpha has been shown to activate pathways involving NF-kappa B and MAPK in an IL-1 R6/IL-1 R rp2-dependent manner.
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Alternate Names
FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A
Gene Symbol
IL36A
Additional IL-36 alpha/IL-1F6 Products
Product Documents for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free
Product Specific Notices for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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