IL-33 Antibody (CL13438) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24558
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL13438
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from O95760, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for IL-33 Antibody (CL13438) - BSA Free
Immunohistochemistry-Paraffin: IL-33 Antibody (CL13438) [NBP3-24558]
Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: IL-33 Antibody (CL13438) [NBP3-24558]
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: IL-33 Antibody (CL13438) [NBP3-24558]
Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
![IL-33 Antibody (CL13438) - BSA Free Immunocytochemistry/ Immunofluorescence: IL-33 Antibody (CL13438) [NBP3-24558]](https://resources.bio-techne.com/images/products/nbp3-24558_-immunocytochemistry-immunofluorescence-639174077065325599.jpg "Immunocytochemistry/ Immunofluorescence: IL-33 Antibody (CL13438) [NBP3-24558]")
Applications for IL-33 Antibody (CL13438) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-33
Long Name
Interleukin 33
Alternate Names
C9orf26, DVS27, IL33, NF-HEV
Gene Symbol
IL33
Additional IL-33 Products
Product Documents for IL-33 Antibody (CL13438) - BSA Free
Product Specific Notices for IL-33 Antibody (CL13438) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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