Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336] - Analysis of IL-21. Full-length recombinant IL-21 protein probed with 1 ug/ml of IL-21 monoclonal antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for IL-21 Antibody (14k5H3) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 27531854)

Immunohistochemistry

reported in scientific literature (PMID 27531854)

Western Blot

1-3ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-21

Interleukin-21, also alternatively named IL-17C, is a pro-inflammatory cytokine and member of the IL-17 family. The IL-17 family is comprised of at least six members: IL-17A-F (1, 2). Interleukin-21, along with IL-17B, appears to stimulate release of TNF and IL-1 from the monocytic THP-1 line thus appearing to have a different receptor and induction pattern than IL-17A (1, 2). As a pro-inflammatory cytokine IL-21 augments the inflammatory process in arthritis models (2, 3). Interleukin-21 is induced in epithelial cells by bacterial antigens and other inflammatory ligands such as imiquimod, exhibiting a direct linkage with innate immune receptors such as TLRs (2, 3). The IL-21 receptor complex appears to be comprised of IL-17RA or IL-17RE. Antibody to IL-21 allows further analysis of the unique regulatory activity, cellular receptors and cellular targets of IL-21 (IL-17C) versus other members of the IL-17 family such as IL-17A. The study of Interleukin-21 and its contribution to diseases caused by impaired epithelial barriers in autoimmune and inflammatory diseases such as psoriasis, asthma or inflammatory bowel disease potentiate development of new therapies directed at regulation of IL-21 or other IL-17 family members (4). The linkage of IL-21 with epithelial cells also furthers the study of the innate immune functions of epithelial cells.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Gene Symbol

IL21

UniProt

Q9HBE4

Additional IL-21 Products

Product Documents for IL-21 Antibody (14k5H3) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for IL-21 Antibody (14k5H3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

IL-21 Antibody (14k5H3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27336

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all IL-21 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for IL-21 Antibody (14k5H3) - BSA Free

Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336]

Applications for IL-21 Antibody (14k5H3) - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: IL-21

Long Name

Alternate Names

Gene Symbol

UniProt

Additional IL-21 Products

Product Documents for IL-21 Antibody (14k5H3) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for IL-21 Antibody (14k5H3) - BSA Free