IgG2c Antibody (RM223) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62013B
Recombinant Monoclonal Antibody
![IgG2c Antibody (RM223) [Biotin]](https://resources.bio-techne.com/images/products/nbp2-62013b_rabbit-monoclonal-igg2c-antibody-rm223-biotin-31-1103-02-elisa-18920252348531.png "ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013B] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM223 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Mouse IgG2c
Specificity
This antibody reacts to the Fc region of mouse IgG2c.No cross reactivity with mouse IgG1, IgG2a, IgG2b,IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG2c Antibody (RM223) [Biotin]
ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013B] -
ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013B] - A titer ELISA of Mouse IgG2c. The plate was coated with different amounts of Mouse IgG2c. A serial dilution of RM223 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.![IgG2c Antibody (RM223) [Biotin]](https://resources.bio-techne.com/images/products/nbp2-62013b_rabbit-monoclonal-igg2c-antibody-rm223-biotin-31-1103-02-elisa-189202523362016.png "ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013B] -")
ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013B] -
ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013B] - ELISA of IgG from BalB/C and C57BL6 shows RM223 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM223 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.Applications for IgG2c Antibody (RM223) [Biotin]
Application
Recommended Usage
ELISA
0.01 ug/ml - 0.2 ug/ml
Western Blot
0.5 ug/ml - 2 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG2c
Additional IgG2c Products
Product Documents for IgG2c Antibody (RM223) [Biotin]
Product Specific Notices for IgG2c Antibody (RM223) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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