ICAM-1/CD54 Antibody (14C11) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # FAB720CL1

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

MAB720

Allophycocyanin

FAB720A

Biotin

FAB720B

DyLight 350

FAB720D

DyLight 405

FAB720E

DyLight 488

FAB720K

DyLight 550

FAB720L

DyLight 594

FAB720M

DyLight 650

FAB720W

DyLight 680

FAB720Y

DyLight 755

FAB720Z

FITC

FAB720F

HRP

FAB720H

Janelia Fluor 525

FAB720JF525

Janelia Fluor 549

FAB720I

Janelia Fluor 646

FAB720J

Janelia Fluor 669

FAB720JF669

mFluor Violet 450 SE

FAB720MFV450

mFluor Violet 500 SE

FAB720MFV500

mFluor Violet 610 SE

FAB720MFV610

PE

FAB720P

PerCP

FAB720C

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # 14C11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ICAM-1/CD54 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain

Specificity

Detects recombinant human (rh)ICAM-1 in direct ELISAs and Western blots. In Western blots, shows no cross-reactivity with rrICAM-1, rhICAM-2, rhICAM-3, rhVCAM-1, and rmDCC.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for ICAM-1/CD54 Antibody (14C11) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for ICAM-1/CD54 Antibody (14C11) [CoraFluor™ 1]

Application

Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (14C11) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for ICAM-1/CD54 Antibody (14C11) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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