IBTK Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88512
![IBTK Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: IBTK Antibody [NBP1-88512]](https://resources.bio-techne.com/images/products/nbp1-88512_-immunocytochemistry-immunofluorescence-639174076717478926.jpg "Immunocytochemistry/ Immunofluorescence: IBTK Antibody [NBP1-88512]")
![Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512]](https://resources.bio-techne.com/images/products/IBTK-Antibody-Immunohistochemistry-Paraffin-NBP1-88512-img0002.jpg "Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IBTK Antibody - BSA Free
Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512]
Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.![Simple Western: IBTK Antibody [NBP1-88512]](https://resources.bio-techne.com/images/products/IBTK-Antibody-Simple-Western-NBP1-88512-img0004.jpg "Simple Western: IBTK Antibody [NBP1-88512]")
Simple Western: IBTK Antibody [NBP1-88512]
Simple Western: IBTK Antibody [NBP1-88512] - Simple Western lane view shows a specific band for IBTK in 0.2 mg/ml of U-251 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: IBTK Antibody [NBP1-88512]](https://resources.bio-techne.com/images/products/IBTK-Antibody-Simple-Western-NBP1-88512-img0005.jpg "Simple Western: IBTK Antibody [NBP1-88512]")
Simple Western: IBTK Antibody [NBP1-88512]
Simple Western: IBTK Antibody [NBP1-88512] - Electropherogram image(s) of corresponding Simple Western lane view. IBTK antibody was used at 1:20 dilution on U-251 lysate(s).Applications for IBTK Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Simple Western
1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251, separated by Size, antibody dilution of 1:20. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251, separated by Size, antibody dilution of 1:20. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IBTK
Alternate Names
Bruton agammaglobulinemia tyrosine kinase inhibitor, BTK-binding protein, BTKI, DKFZP564B116, IBtk, inhibitor of Bruton agammaglobulinemia tyrosine kinase, inhibitor of Bruton tyrosine kinase, inhibitor of Bruton's tyrosine kinase, inhibitor of Bruton's tyrosine kinase-alpha, inhibitor of Bruton's tyrosine kinase-beta, inhibitor of Bruton's tyrosine kinase-gamma, KIAA1417, MGC142256, MGC142258, RP1-93K22.1
Gene Symbol
IBTK
Additional IBTK Products
Product Documents for IBTK Antibody - BSA Free
Product Specific Notices for IBTK Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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