Scientific Data Examples for Human VISTA/B7-H5/PD-1H Antibody
Western Blot
Detection of Human VISTA/B7-H5/PD-1H by Western Blot.
Western Blot shows lysates of human placenta. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VISTA/B7-H5/PD-1H at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Simple Western
Detection of Human VISTA/B7-H5/PD-1H by Simple WesternTM.Left: Simple Western lane view shows lysates of human placenta, loaded at 0.1 mg/ml. A specific band was detected for VISTA/B7-H5/PD-1H at approximately 59 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) followed by HRP-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in human placenta.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator
Background: VISTA/B7-H5/PD-1H
Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.
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