Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 349406

View all Tryptase alpha/beta-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase alpha/beta-1
Ile31-Pro275
Accession # AAD13876

Specificity

Detects human Tryptase alpha/beta-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50‑100% cross-reactivity with recombinant human (rh) TPSAB1 and recombinant mouse (rm) Mcpt6 is observed, 20‑100% cross-reactivity with rmMcpt7 is observed, and no cross-reactivity with rhTPSG1, rmTPS5, rmMcpt1, or rmMcpt11 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Tryptase alpha/beta-1 Antibody

Detection of Tryptase alpha/beta-1 in Human Lung.

Tryptase alpha/beta-1 was detected in immersion fixed paraffin-embedded sections of Human Lung using Mouse Anti-Human Tryptase alpha/beta-1 Monoclonal Antibody (Catalog # MAB3796) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to mast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Tryptase alpha/beta-1 Antibody

Application
Recommended Usage

Immunohistochemistry

0.1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Lung

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796‑SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796-SE)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase alpha/beta-1

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). They are stored in secretory granules of mast cells, where they form active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, beta tryptases are resistant to macromolecule protease inhibitors (2). When mast cells are activated, beta tryptases are released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). beta tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic disorders.

References

  1. Caughey, G. H. 2004, in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1535.
  2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
  3. Hallgren, J. and G. Pejler (2006) FEBS J. 273:1871.  

Alternate Names

TPS2, TPSB2, Tryptase beta-2, Tryptase beta2, Tryptase-C

Entrez Gene IDs

7177 (Human); 100503895 (Mouse); 54271 (Rat)

Gene Symbol

TPSB2

UniProt

AAD13876

Additional Tryptase alpha/beta-1 Products

Product Documents for Human Tryptase alpha/beta-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Tryptase alpha/beta-1 Antibody

For research use only

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