Troponin T was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Troponin T in Human Heart.

Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Troponin T (Cardiac) Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart tissue

Reviewed Applications

Read 3 reviews rated 5 using MAB1874 in the following applications:

Flow Cytometry (1 Review)
Immunocytochemistry/Immunofluorescence (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin T

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Alternate Names

CMH2, CMPD2, cTnT, TNNT2, TnTC

Entrez Gene IDs

7139 (Human)

Gene Symbol

TNNT2

Additional Troponin T Products

Product Documents for Human Troponin T (Cardiac) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Troponin T (Cardiac) Antibody

For research use only

Citations
Reviews

Human Troponin T (Cardiac) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1874

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Troponin T (Cardiac) Antibody

Troponin T in BG01V Human Embryonic Stem Cells.