In the presence of Recombinant Mouse M-CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in RAW 264.7 mouse macrophages in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) activity in cell lysates. Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/ TNFSF11/RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB626). The ND₅₀ is typically 0.8-2.5 µg/mL.

TRANCE/TNFSF11/RANK L in Human Lymph Node.

TRANCE/TNFSF11/RANK L was detected in immersion fixed paraffin-embedded sections of human lymph node using 5 µg/mL Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB626) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human TRANCE/TNFSF11/RANK L Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Neutralization

Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in RAW 264.7 mouse macrophages. The Neutralization Dose (ND₅₀) is typically 0.8-2.5 µg/mL in the presence of 30 ng/mL Recombinant Human TRANCE/TNFSF11/RANK L.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB626 in the following applications:

ELISA (1 Review)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TNF-related activation-induced cytokine (TRANCE; also RANKL, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2 lacks aa 1-73, and isoform 3 lacks aa 1-47. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart,placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

UniProt

O14788

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Human TRANCE/TNFSF11/RANK L Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TRANCE/TNFSF11/RANK L Antibody

For research use only

Citations
Reviews

Human TRANCE/TNFSF11/RANK L Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB626

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human TRANCE/TNFSF11/RANK L Antibody

Osteoclast-Like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody.