Human TR alpha/NR1A1 Antibody

Detection of TR alpha /NR1A1 by Western Blot

Progestins enhanced the growth of THRB-silenced EC cells. (A) THRA or THRB silencing efficiency in RL95-2 and KLE cells. (B,C) Protein expression of TR alpha or TR betasilencing in RL95-2 and KLE cells. (D,G) Cell viability after silencing THRA or THRB in RL95-2 and KLE cells. The cells were treated with si-THRA or si-THRB for 48 h and then cultured in fresh media for 24, 48, 72, and 96 h. (E,F) RL95-2 or (H,I) KLE cells were pretreated with si-THRA or si-THRB for 48 h and then treated with 30 μM MPA (E,H) or NOMAc (F,I); meanwhile, 100 nM T3 was added for 48 h to examine cell viability. The cell viability was normalized to the control, which was set at 100%. The results are presented as mean ± SEM of three independent experiments. TR alpha/THRA, thyroid hormone receptor alpha; TR beta/THRB: thyroid hormone receptor beta; si-Ctrl, negative control treated with siRNA solvent; si-THRA, silenced THRA; si-THRB, silenced THRB. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36293372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of TR alpha /NR1A1 by Western Blot

Progestins enhanced the growth of THRB-silenced EC cells. (A) THRA or THRB silencing efficiency in RL95-2 and KLE cells. (B,C) Protein expression of TR alpha or TR betasilencing in RL95-2 and KLE cells. (D,G) Cell viability after silencing THRA or THRB in RL95-2 and KLE cells. The cells were treated with si-THRA or si-THRB for 48 h and then cultured in fresh media for 24, 48, 72, and 96 h. (E,F) RL95-2 or (H,I) KLE cells were pretreated with si-THRA or si-THRB for 48 h and then treated with 30 μM MPA (E,H) or NOMAc (F,I); meanwhile, 100 nM T3 was added for 48 h to examine cell viability. The cell viability was normalized to the control, which was set at 100%. The results are presented as mean ± SEM of three independent experiments. TR alpha/THRA, thyroid hormone receptor alpha; TR beta/THRB: thyroid hormone receptor beta; si-Ctrl, negative control treated with siRNA solvent; si-THRA, silenced THRA; si-THRB, silenced THRB. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36293372), licensed under a CC-BY license. Not internally tested by R&D Systems.