Human TRA-1-60(R) PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4770P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgM Clone # TRA-1-60
Product Specifications
Immunogen
2102Ep human embryonal carcinoma cell line
Specificity
Detects human TRA‑1‑60(R).
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for Human TRA-1-60(R) PE-conjugated Antibody
Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60(R) PE-conjugated Monoclonal Antibody (Catalog # FAB4770P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human TRA-1-60(R) PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: BGO1V Human Embryonic Stem Cells
Sample: BGO1V Human Embryonic Stem Cells
Reviewed Applications
Read 1 review rated 5 using FAB4770P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-60(R)
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2-4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).
References
- Andrews, P. et al. (1984) Hybridoma 3:347.
- Thomson, J. et al. (1998) Science 282:1145.
- Giwercman, A. et al. (1993) Cancer 72:1308.
- Marrink, J. et al. (1991) Int. J. Cancer 49:368.
- Badcock, G. et al. (1999) Cancer Res. 59:4715.
- Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Long Name
Tumor-related Antigen-1-60 [R]
Alternate Names
TRA160(R)
Entrez Gene IDs
5420 (Human)
Gene Symbol
PODXL
Additional TRA-1-60(R) Products
Product Documents for Human TRA-1-60(R) PE-conjugated Antibody
Product Specific Notices for Human TRA-1-60(R) PE-conjugated Antibody
For research use only
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