Detects human TNF RII/TNFRSF1B in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human TNF RI, recombinant mouse (rm) TNF RI, and rmTNF RII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TNF RII/TNFRSF1B Biotinylated Antibody

Human TNF RII / TNFRSF1B ELISA Standard Curve

Recombinant Human TNF RII/TNFRSF1B aa 24-206 (Catalog # 1089-R2) was serially diluted and captured by Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TNF RII/TNFRSF1B Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2)

Human TNF RII/TNFRSF1B Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human TNF RII/TNFRSF1B Antibody (Catalog # MAB726)
Standard: Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein (Catalog # 1089-R2)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF726 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RII/TNFRSF1B

TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

Additional TNF RII/TNFRSF1B Products

Product Documents for Human TNF RII/TNFRSF1B Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TNF RII/TNFRSF1B Biotinylated Antibody

For research use only

Citations
Reviews

Human TNF RII/TNFRSF1B Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF726

Key Product Details

Species Reactivity

Validated:

Cited:

Applications