Human Thymosin beta4 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6796G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Thymosin β4
Ser2-Ser44
Accession # P62328
Ser2-Ser44
Accession # P62328
Specificity
Detects human Thymosin beta4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Thymosin beta10 and rhThymosin beta16 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Thymosin beta 4
Thymosin beta 4 (T beta4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. Members of this family range from 41-44 amino acids (aa) in length, and possess an isoelectric point that lies between pH 4.0-7.0 ( alpha-thymosins have values less than 4.0). Multiple cell types produce T beta4, either constitutively, or after stimulation. They include platelets, endothelial cells, neutrophils, astrocytes and macrophages. T beta4 is both a secreted and intracellular molecule. The secreted form contributes to wound healing and angiogenesis, and may act on ATPase. Intracellularly, it forms a 1:1 complex with G-actin and blocks F-actin polymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration. Mature human T beta4 is 43 aa in length (aa 2-44). It contains an actin-binding site (aa 17-23), one acetylated Ser and five acetylated lysines (4; 12; 26; 32; 39) and one phosphorylation site at Thr23. T beta4 undergoes proteolytic processing to generate an N-terminal acetylated peptide (aa 2-5: SerAspLysPro). Mature human T beta4 is identical to mouse T beta14 in aa sequence, and it shares 74% aa identity with its human family member T beta10.
Additional Thymosin beta 4 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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