Human TGR5/GPBAR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4286
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, CyTOF-ready
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 409522
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1
Met1-Asn330
Accession # Q8TDU6
Met1-Asn330
Accession # Q8TDU6
Specificity
Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TGR5/GPBAR1 Antibody
Detection of TGR5/GPBAR1 in HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) human TGR5/GPBAR1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TGR5/GPBAR1 Monoclonal Antibody (Catalog # MAB4286) followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on Mouse IgG2BIsotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.Applications for Human TGR5/GPBAR1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP
Sample: HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGR5/GPBAR1
G protein coupled bile acid receptor 1 (GPBAR1), also called M-BAR, BG37 or TGR5, is a G-protein coupled receptor superfamily member that is a widely expressed cell surface receptor for bile acids. On macrophages, bile acid engagement of GPBAR1 suppresses phagocytosis and other functions. Extracellular portions of human GPBAR1 show approximately 75% amino acid identity with corresponding regions of mouse GPBAR1.
Long Name
Bile Acid Receptor TGR5
Alternate Names
BG37, GPBA, GPBAR1, GPCR19, GPR131, M-BAR
Gene Symbol
GPBAR1
UniProt
Additional TGR5/GPBAR1 Products
Product Documents for Human TGR5/GPBAR1 Antibody
Product Specific Notices for Human TGR5/GPBAR1 Antibody
For research use only
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