TCF-2/HNF-1 beta was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red;NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

TCF‑2/HNF‑1 beta in Human Kidney.

TCF-2/HNF-1 beta was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

TCF‑2/HNF‑1 beta in Rat Kidney.

TCF-2/HNF-1 beta was detected in perfusion fixed frozen sections of rat kidney using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human TCF-2/HNF-1 beta Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and BG01V human embryonic stem cells differentiated into pancreatic progenitor cells

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and perfusion fixed frozen sections of rat kidney

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-2/HNF-1 beta

TCF-2, also known as HNF-1 beta (hepatocyte nuclear factor 1 beta) and VHNF-1, is a member of the HNF-1 homeobox family. By alternative splicing, three isoforms of TCF-2/HNF-1 beta exist. TCF-2/HNF-1 beta binds DNA as a homodimer or heterodimer with TCF-1/HNF-1 alpha. Within the region used as the immunogen, human TCF-2 shares 98% amino acid sequence homology with mouse and rat TCF-2. TCF-2 expression is required for visceral endoderm specification.

Long Name

Transcription Factor 2

Alternate Names

HNF-1 beta, HNF1B, HNF2, LF-B3, MODY5, TCF2, VHNF1

Entrez Gene IDs

6928 (Human); 21410 (Mouse); 25640 (Rat)

Gene Symbol

HNF1B

UniProt

P35680

Additional TCF-2/HNF-1 beta Products

Product Documents for Human TCF-2/HNF-1 beta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TCF-2/HNF-1 beta Antibody

For research use only

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