Human TAZ/WWTR1 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB7210S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 672027
Product Specifications
Immunogen
E. coli-derived recombinant human TAZ/WWTR1
Met267-Leu400
Accession # Q9GZV5
Met267-Leu400
Accession # Q9GZV5
Specificity
Detects human TAZ/WWTR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) YAP or rhYes is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human TAZ/WWTR1 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: TAZ/WWTR1
TAZ (Transcriptional co-Activatorr with PDZ-binding motif; also WWTR1) is a 50-55 kDa protein that is related to YAP65 TEF-1 interacting protein. It is a widely expressed transcriptional coactivator, and should not be confused with tafazzin/Taz, an enzyme associated with lipid metabolism. TAZ influences the nuclear transport of SMAD-2, -3 and -4, and in the nucleus, TAZ is known to interact with transcription factors such as TFF1, Pax8, NKX2-1 and TEADS, serving as a scaffold for transcriptional activation complexes. Human TAZ is 400 amino acids (aa) in length. It contains one WW domain (aa 124-157) that binds to PPXY motifs, a coiled-coil region (aa 225-259), and a PDZ binding domain (aa 394-400). There are at least four utilized phosphorylation sites. When phosphorylated on Ser89, TAZ preferentially bind to 14-3-3 proteins, promoting its retention in the cytoplasm. Over aa 267-400, human TAZ shares 88% aa identity with mouse TAZ.
Long Name
Transcriptional Coactivator with PDZ-binding Motif
Alternate Names
WWTR1
Gene Symbol
WWTR1
UniProt
Additional TAZ/WWTR1 Products
Product Specific Notices for Human TAZ/WWTR1 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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