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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 705129

Product Specifications

Immunogen

E. coli-derived recombinant human STIM1
Leu23-Thr182
Accession # Q13586

Specificity

Detects human STIM1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human STIM2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human STIM1 Alexa Fluor® 350-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: STIM1

STIM-1 (stromal interaction molecule 1), previously called GOK, is a 90 kDa type I transmembrane protein of the endoplasmic reticulum (ER). When STIM-1 senses depletion of calcium in the ER via its EF-hand domain (aa 63-98), it interacts with the plasma membrane Ca2+ release-activated Ca2+ (CRAC) channel Orai1, increasing Ca2+ influx. The human STIM-1 extracellular/lumenal domain (aa 23-213) contains an EF hand Ca2+-binding motif and a SAM (sterile a-motif) multimerization domain. A potential isoform is truncated at aa 491. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2). Within the region used as an immunogen, human STIM1 shares 95% amino acid identity with mouse and rat STIM1.

Long Name

Stromal Interaction Molecule 1

Alternate Names

GOK

Entrez Gene IDs

6786 (Human); 20866 (Mouse); 361618 (Rat)

Gene Symbol

STIM1

UniProt

Additional STIM1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human STIM1 Alexa Fluor® 350-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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