Detects human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human ST3GAL2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

Detection of Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 by Western Blot.

Western blot shows lysates of human placenta. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST3 beta-Gal a-2,3-Sialyltransferase 1/ST3GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta-Gal a-2,3-Sialyltransferase 1/ ST3GAL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Human placenta

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1

ST3 beta-Gal alpha-2,3-Sialyltransferase 1 (ST3GAL1, also known as SIAT4 and SIAT4A) is a 45 kDa member of glycosyltransferase family 29 that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The full length ST3GAL1is a type II Golgi membrane glycoprotein that can be proteolytically processed to a soluble form. ST3Gal1 contributes to the alpha2-3-sialylation of O-glycans such as T antigens. Upon maturation of Dendritic cells derived from monocytes ST3GAl1 is altered in a stimulus-dependent manner. Multiple isoforms of human ST3GAL1 have been described. Human ST3GAL1 shares 85% identity with mouse ST3GAL1.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 1

Alternate Names

Gal-NAc6S, SIAT4A, SIATFL, ST3GalA, ST3O

Entrez Gene IDs

6482 (Human); 20442 (Mouse); 362924 (Rat)

Gene Symbol

ST3GAL1

UniProt

Q11201

Additional ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Products

Product Documents for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

For research use only

Citations
Reviews

Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6905

Key Product Details

Species Reactivity

Validated:

Cited:

Applications