Somatostatin was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Rabbit Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB11635) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm . View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Somatostatin Antibody

Application

Recommended Usage

Immunohistochemistry

1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human hypothalamus

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Somatostatin

Somatostatin (SST), also known as SMST, is a 116 amino acid (aa) peptide hormone protein and shares 96% aa identity with mouse and rat SST. It exists as a preproprotein that is cleaved into a proprotein form and then subsequently into 14 aa and 28 aa active peptide forms. SST is secreted by many tissues throughout the body and inhibits the secretion of many other hormones. Some of its major roles are inhibiting growth hormone secretion by the pituitary gland, insulin and glucagon secretion in pancreatic islets, and secretion of gastrointestinal tract hormones such as gastrin. Additionally, SST has been shown to have neuromodulatory activity.

Alternate Names

SMST, SOM, SRIF, SST

Entrez Gene IDs

6750 (Human); 20604 (Mouse); 24797 (Rat)

Gene Symbol

SST

UniProt

P61278

Additional Somatostatin Products

Product Documents for Human Somatostatin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Somatostatin Antibody

For research use only

Citations
Reviews

Human Somatostatin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11635

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Somatostatin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Somatostatin Antibody

Detection of Somatostatin in Human Hypothalamus.